Publications
 

Home Research Projects Lab Personnel Other Lab Activities Publications

Recent Publications:

  1. Mattern, H.M. and Hardin, C.D. (2007).  Lipotoxicity in vascular smooth muscle.  In press, Physiological Research

  2. Raikar LS, Vallejo J, Lloyd PG, Hardin CD. (2006).  Overexpression of caveolin-1 results in increased plasma membrane targeting of glycolytic enzymes: The structural basis for a membrane associated metabolic compartment. J Cell Biochem.  Feb 1; (in Press)

  3. Vallejo J, Hardin CD. (2006).  Caveolins in vascular smooth muscle: Form organizing function.
    Cardiovasc Research. 69(4):808-15. (in Press)

  4. Vallejo J, Hardin CD. (2005) Expression of caveolin-1 in lymphocytes induces caveolae formation and recruitment of phosphofructokinase to the plasma membrane. FASEB Journal. 19(6):586-7. Epub 2005 Jan 21.

  5. Vallejo J, Hardin CD. (2004) Caveolin-1 functions as a scaffolding protein for phosphofructokinase in the metabolic organization of vascular smooth muscle. Biochemistry.  43(51):16224-32.

  6. Vallejo J, Hardin CD. (2003).  Metabolic organization in vascular smooth muscle: distribution and localization of caveolin-1 and phosphofructokinase. American Journal of Physiology.Cell Physiology. 286(1):C43-54. Epub 2003 Aug 27.

  7. Hardin CD, Kleiber BD, Roberts TM. (2003).  Mitochondrial oxidative substrate selection in porcine bladder smooth muscle. The Journal of Urology. 170(5):2063-6.

  8. Greenwalt TJ, Gormas JF, Rugg N, Rios J, Goldsmith JC, Prokopczuk E, Hardin CD, Roberts TM. (2002).  Evaluation of fructose diphosphate in RBC preservation. Transfusion. 42(3):384-5.

  9. Hardin CD, Lazzarino G, Tavazzi B, Di Pierro D, Roberts TM, Giardina B, Rovetto MJ. (2001).  Myocardial metabolism of exogenous FDP is consistent with transport by a dicarboxylate transporter. American Journal of Physiology. Heart and Circulatory Physiology. 281(6):H2654-60.

  10. Allen TJ, Hardin CD. (2001).  Oleate oxidation and mitochondrial substrate selection in vascular smooth muscle. Journal of Vascular Research. 38(3):276-87.

  11. Lloyd PG, Hardin CD. (2001).  Caveolae and the organization of carbohydrate metabolism in vascular smooth muscle.  Journal of Cellular Biochemistry. 82(3):399-408.

  12. Roberts TM, Sturek M, Dixon JL, Hardin CD. (2001).  Alterations in the oxidative metabolic profile in vascular smooth muscle from hyperlipidemic and diabetic swine.  Molecular and Cellular Biochemistry. 217(1-2):99-106.

  13. Allen, T.J. and Hardin, C.D.  (2000). The influence of glycogen storage on vascular smooth muscle metabolism.  American Journal of Physiology: Heart and Circulatory Physiology. 278: H1993-2002.

  14. Lloyd, P.G., and Hardin, C.D. (2000). Sorting of metabolic pathway flux by the plasma membrane in cerebrovascular smooth muscle cells.  American Journal of Physiology: Cell Physiology. 47: C803-811.

  15. Lloyd, P.G., and Hardin, C.D. (1999).  The role of microtubules in the regulation of metabolism in isolated cerebral microvessels. American Journal of Physiology: Cell Physiology 277: C1250-1262.

  16. Lloyd, P.G., Hardin, C.D., and Sturek, M. (1999).  Examining glucose transport in single vascular smooth muscle cells with a fluorescent glucose analog. Physiological Research 48: 401-410.

  17. Finder, D.R. and Hardin, C.D. (1999).  Transport and metabolism of exogenous fumarate and 3-phosphoglycerate in vascular smooth muscle. Molecular and Cellular Biochemistry 195:113-121.

  18. Allen, T.M. and Hardin, C.D.  (1998).  The pattern of substrate utilization in vascular smooth muscle using 13C-isotopomer analysis of glutamate. American Journal of Physiology: 275(44): H2227-H2235.

  19. Hardin, C.D. and Finder, D.R. (1998).  Glycolytic flux in permeabilized freshly isolated vascular smooth muscle cells.  American Journal of Physiology: 274(43): C88-C96.

  20. Gann V.K. and Hardin, C.D.  (1997).  Acetate or octanoate increases glycogenolysis in smooth muscle as determined by 13C-NMR.  Physiological Chemistry and Physics and Medical NMR  29: 23-32.

  21. Hardin, C.D. and Roberts, T.M.  (1997).  Differential regulation of glucose and glycogen metabolism in vascular smooth muscle by exogenous substrates.  Journal of Molecular and Cellular Cardiology 29: 1207-1216.

  22. Hardin, C.D. and Roberts, T.M.  (1997).  Regulation of glycogen utilization, but not glucose utilization, by pre-contraction glycogen levels in vascular smooth muscle.  Biochemistry 36(23): 6954-6959.

  23. Dykens, J.A., Wiseman, R.W., and Hardin, C.D.  (1996).  Preservation of phosphagen kinase function during transient hypoxia via enzyme abundance or resistance to oxidative inactivation.  Journal of Comparative Physiology 166:359-368.

  24. Juergens, T.M. and Hardin, C.D.  (1996).  Fructose-1,6-bisphosphate as a metabolic substrate in hog ileum smooth muscle during hypoxia.  Molecular and Cellular Biochemistry 154:83-93.

  25. Hardin, C.D. and Roberts, T.M.  (1995).  Compartmentation of glucose and fructose-1,6-bisphosphate metabolism in vascular smooth muscle.  Biochemistry  34(4): 1323 1331.

  26. Hardin, C.D. and Roberts, T.M.  (1995).  Gluconeogenesis During Hypoxia in Vascular Smooth Muscle Studied by 13C-NMR.  Physiological Research 44:257-260.

  27. Hardin C.D., Kushmerick, M.J., and Roberts, T.M.  (1995).  Vascular smooth muscle glycogen metabolism studied by 13C-NMR.  Journal of Vascular Research  32:293-300.

  28. Hardin, C.D. and Roberts, T.M.  (1994).  The metabolism of exogenously applied fructose-1,6-bisphosphate in hypoxic vascular smooth muscle. American Journal of Physiology 267(36): H2325-2332.

  29. Hardin, C.D., and Kushmerick, M.J.  (1994).  Simultaneous and separable flux of pathways for glucose and glycogen utilization studied by 13C-NMR.  Journal of Molecular and Cellular Cardiology 26: 1197-1210.

  30. Hardin C.D., Paul, R.J., Raeymaekers, L., Steenart N.A.E., and Kranias, E.G. (1993).  Regulation of glycolytically fueled Ca2+-uptake in smooth muscle plasmalemmal vesicles by phosphorylation.  American Journal of Physiology.  263(34): H1326-H1333.

  31. Hardin, C.D., Wiseman, R.W., and Kushmerick, M.J. (1992).  Vascular oxidative metabolism under different metabolic conditions.  Biochimica et Biophysica Acta  1133: 133-141.

  32. Skubatch, H., Hardin, C.D., Wiseman, R.W., Meeuse, B.J.D., and Kushmerick, M.J. (1992).  The energetic state of the thermogenic appendix of the voodoo lily inflorescence: A 31P NMR study. Biochimica et Biophysica Acta 1100: 98-103.

  33. Hardin, C.D., Raeymaekers, F., and Paul, R.J.  (1992).  Comparison of endogenous and exogenous sources of ATP in supporting Ca2+-uptake in isolated smooth muscle plasma membrane vesicles (PMV).  Journal of General Physiology.  99: 21-40.

  34. Hardin, C.D., Wiseman, R.W., and Kushmerick, M.J.  (1992).  Tension responses of sheep aorta to simultaneous decreases in phosphocreatine, inorganic phosphate, and ATP.  Journal of Physiology (Lond.)  458: 139-150.

  35. Hardin, C.D., and Paul, R.J.  (1992).  Localization of two glycolytic enzymes in guinea pig taenia coli.  Biochimica et Biophysica Acta 1134: 256-259.

  36. Paul, R.J., Hardin, C.D., Raeymaekers, L., Wuytack, F., and Casteels, R.  (1989).  An endogenous glycolytic cascade can preferentially support Ca2+-uptake in smooth muscle plasma membrane vesicles. FASEB Journal 3: 2299-2301.

 

2006 under review:

      1)      Mattern, H.M., Lloyd. P.G., Sturek, M., and Hardin, C.D.  (2007). Gender and genetic differences in bladder smooth muscle mRNA in a porcine model of the metabolic syndrome

 

 

Invited Papers/Book Chapters:

1)     Hardin C.D.  (2001).  Making sense of oxygen sensing.  Journal of Physiology 536: 1.

2)   Hardin, C.D., Allen, T.J., and Paul, R.J. (2001).  Metabolism and energetics of vascular smooth muscle.  In: Physiology and Pathophysiology of the Heart., 4th Edition, N. Sperelakis (ed)., Kluwer Academic Publishers, Norwell, MA.  pp 571-595.

3)   Hardin, C.D. and Paul, R.J. (1995).  Metabolism and energetics of vascular smooth muscle.  In: Physiology and Pathophysiology of the Heart., 3rd Edition, N. Sperelakis (ed)., Kluwer Academic Publishers, Norwell, MA.  pp 1069-1086.

4)   Paul, R.J., Hardin, C.D., Campbell, J., and Raeymaekers, L. (1989).  Compartmentation of Metabolism and Function in Smooth Muscle, in Muscle Energetics (Paul, Elzinga, and Yamada, eds.), pp. 381-390, Alan R. Liss, New York.

 

Abstracts:

1)   Mattern, H.M., Raikar, L.S., and Hardin, C.D. (2006)  The effect of caveolin-1 (CAV-1) on fatty acid uptake and CD36 localization in vascular smooth muscle (VSM) cells  FASEB J. (in press)

2)   Hernandez, M., Raikar, L.S., Roberts, T.M, and Hardin, C.D.  (2006)  Localization of glycolysis to the plasma membrane can occur by a caveolin-1 mediated mechanism.  Biophys. J. (in press)

3)   Hardin, C.D.  (2005) Structural basis of glycolytic compartmentation: a cytoarchitect’s guide to metabolism.  The FEBS Journal (in press)

4)   Hardin, C.D., Raikar, L.S., Lloyd, P.G., and Roberts, T.M.  (2005)  Overexpression of caveolin-1 results in increased plasma membrane targeting of phosphofructokinase (PFK): The structural basis for a membrane associated metabolic compartment.  FASEB J. 19(4): A273.

5)   Vallejo, J., Roberts, T.M., and Hardin, C.D.  (2005) New caveolin-1 expression in lymphocytes functions as a scaffold for glycolysis by recruitment of phosphofructokinase to the plasma membrane.  FASEB J. 19(4): A663.

6)  Hardin, C.D., Lloyd, P.G., Mattern, H.M., Stricklin, C., Sturek, M., and Roberts, T.M.  (2005) Smooth muscle caveolin-1 mRNA levels are reduced in a genetic model of the metabolic syndrome and modulated by high fat diets.  FASEB J. 19(4): A195.

7)  Roberts, T.M. and Hardin, C.D.  (2005)  Metabolic fuel switching is limited in vascular smooth muscle (VSM): a role for PDK3?  FASEB J. 19(4): A632.

8)  Mattern, H.M., Lloyd, P.G., Sturek, M., and Hardin, C.D. (2005)  Gender and genetic differences in bladder smooth muscle PPAR mRNA in a porcine model of the metabolic syndrome.  FASEB J 19(4): A260.

9)   Mattern, H.M., Allen, T.J., Roberts, T.M., and Hardin, C.D.  (2005)  Lipotoxicity in vascular smooth muscle (VSM).  FASEB J. 19(4):  A699.

10) Hardin, C.D., Petersen, A., Sturek, M. and Roberts, T.M.  (2005)  Bladder smooth muscle from diabetic/dyslipidemic swine exhibit altered phenotype and metabolic dysfunction: pathogenesis of lipotoxicity in smooth muscle.  FASEB J. 19(4): A124.

11) Vallejo, J. and Hardin, C.D. (2004).  Caveolin-1 (CAV-1) functions as a scaffolding protein for phosphofructokinase (PFK) in the metabolic organization of vascular smooth muscle (VSM)  Late breaking abstract to EB 2004

12) Vallejo, J. and Hardin, C.D. (2003).  Smooth muscle metabolic organization: a role for caveolar domains of the plasma membrane. FASEB J.

13) Peterson, A., Roberts, T.M., Sturek, M., Dixon, J.L., and Hardin, C.D. (2003).  Bladder smooth muscle phenotype alterations in diabetic/dyslipidemic swine:  a role for lipotoxicity?  FASEB J.

14) Hood, J., Roberts, T.M., Rovetto, M.J., and Hardin, C.D. (2003).  Preferential oxidation of exogenous fructose bisphosphate:  novel channeling to the mitochondria?  Late breaking abstract to EB 2003.

15) Vallejo, J. and Hardin, C.D. (2002).  Metabolic Organization in Vascular Smooth Muscle (VSM): Distribution and Localization of Caveolin-1 (CAV-1) and Phosphofructokinase (PFK).  FASEB J. 16(4):A97.

16) Hardin C.D. (2001).  Redox and metabolism in the vascular wall: an overview.  J. Mol. Cell. Cardiol. 33(6) A157.

17) Vallejo, J. and Hardin, C.D. (2001).  Localization of caveolin-1 and phosphofructokinase and metabolic organization in vascular smooth muscle.  J. Mol. Cell. Cardiol. 33(6) A125.

18) Hollenbeck, A.C., Roberts, T.M., and Hardin C.D. (2001).  Type II diabetes-induced alterations in mitochondrial substrate utilization may be due to effects of palmitate.  J. Mol. Cell. Cardiol. 33(6) A48.

19) Allen, T.J. and Hardin, C.D. (2001).  Oxidation and storage of long-chain lipid (oleate) in vascular smooth muscle.   J. Mol. Cell. Cardiol. 33(6) A161.

20) Lloyd, P.G. and Hardin, C.D. (2000).  Caveolae and metabolic organization. FASEB J. 14(4): A149.

21) Allen, T.J. and Hardin, C.D.  (2000).  The influence of glycogen storage on vascular smooth muscle metabolism.  FASEB J. 14(4): A149.

22) Roberts, T.M., Kleiber, B.D., and Hardin, C.D. (1999).  13C-Isotopomer analysis of substrate utilization in porcine bladder.  FASEB J. 13(5):A902.

23) Lloyd, P.G. and Hardin, C.D. (1999).  Channeling and organization of glycolysis and gluconeogenesis in permeabilized pig cerebral microvessels (PPCMV).  FASEB J. 13(5):A33.

24) Lloyd, P.G. and Hardin, C.D. (1999).  The role of microtubules in the regulation of glycolysis in freshly isolated pig cerebral microvessels (PCMV). Biophys. J. 76(1): A42.

25) Hardin, C.D., Giardina, B., Tavazzi, B., DiPierro, D., Lazzarino, G., Galvano, M., and Rovetto, M.J. (1998).  Transport mechanism of fructose 1,6-bisphosphate in isolated rat hearts. J. Mol. Cell. Cardiol. 30: A62.

26) Roberts, T.M., Sturek, M., Dixon, J., Weisman, G.A., and Hardin, C.D. (1998).  Substrate utilization in carotid arteries from normal, diabetic/hyperlipidemic, and hyperlipidemic pigs. J. Mol. Cell. Cardiol. 30: A109.

27) Lloyd, P.G., A.D. Thompson, and Hardin, C.D. (1998). Examining the structural basis of compartmented glycolysis in pig cerebral microvessels. J. Mol. Cell. Cardiol. 30: A122.

28) Lloyd, P.G., Hardin, C.D., and M. Sturek. (1998). Use of a small fluorescent glucose analog as an indicator of glucose transport in pig vascular smooth muscle cells. J. Mol. Cell. Cardiol. 30: A63.

29) Finder, D.R. and Hardin, C.D.  (1998).  Glyconeogenesis from proglycogen in vascular smooth muscle (VSM). J. Mol. Cell. Cardiol. 30: A123.

30) Jeffrey, T.M., and Hardin, C.D.  (1998).  The pattern of substrate utilization in vascular smooth muscle using 13C-isotopomer analysis of glutamate.  J. Mol. Cell. Cardiol. 30: A160.

31) Finder, D.R. and Hardin, C.D.  (1998). Transport of exogenous fumarate and 3-phosphoglycerate (3-PG) by a dicarboxylate transporter. J. Mol. Cell. Cardiol. 30: A123.

32) Hardin, C.D., Roberts, T.M., Finder, D.R., Lloyd, P.G., and Jeffrey T.M. (1998).  Organization and integration of metabolism in vascular smooth muscle (VSM) assessed by 13C-NMR.  Biophys. J. 74(2): A224.

33) Finder, D.R. and Hardin, C.D.  (1997).  Exogenous 13C-fumarate metabolism in hypoxic vascular smooth muscle (VSM).  J. Mol. Cell. Cardiol. 29(6): A191.

34) Finder, D.R. and Hardin, C.D.  (1997).  Glycolytic flux in permeabilized freshly isolated hog carotid vascular smooth muscle cells.  J. Mol. Cell. Cardiol. 29(6): A190.

35) Hardin, C.D. and Roberts, T.M.  (1997).  Influence of glycogen levels on glycogen and glucose utilization in contracting vascular smooth muscle.  J. Mol. Cell. Cardiol. 29(6): A191.

36) Hardin, C.D. and Roberts, T.M.  (1996).  Modulation of glucose and glycogen metabolism in vascular smooth muscle (VSM) by alternate exogenous substrates studied by 13C-NMR.  FASEB J.  10(3):  A725.

37)  Hardin, C.D. and Roberts, T.M.  (1995).  Compartmentation of glycolysis and gluconeogenesis in vascular smooth muscle.  FASEB J. 9(3): A315.

38)  Juergens, T.M. and Hardin, C.D.  (1995).  The protective role and metabolism of fructose-1,6-bisphosphate in hog ileum smooth muscle during hypoxia.  FASEB J. 9(3):A422.

39)  McJunkin, T.L., Ewen, A.J., Roberts, T.M., and Hardin, C.D. (1995).  13C-NMR studies of glycolytic metabolism in freshly isolated vascular smooth muscle cell suspensions.  FASEB J. 9(3):A608.

40)  Gann, V., Roberts, T.M., Edwards, E., and Hardin, C.D. (1995).  Effects of short-chain fatty acid utilization on glucose and glycogen utilization in hog carotid artery studied by 13C-NMR.  FASEB J. 9(3):A608.

41)  Hardin, C.D., and Roberts, T.M.  (1994).  Fructose 1,6-bisphosphate (FDP) is metabolized by hypoxic vascular smooth muscle, as determined by 13C-NMR, but does not improve maintenance of isometric force.  FASEB J. 8(4): A556.

42)  Hardin, C.D., and Kushmerick, M.J.  (1992).  13C-NMR Assessment of the dynamics and order of vascular glycogen synthesis and mobilization.  J. Mol. Cell. Cardiol. 24:S25.

43)  Wiseman, R.W., Hardin, C.D., and Dykens, J.A. (1992).  Dynamics of PCr recovery following hypoxia in smooth muscle.  J. Mol. Cell. Cardiol. 24:S25.

44)  Hardin, C.D., and Kushmerick, M.J. (1992).  Simultaneous and separable flux of pathways for glucose and glycogen utilization in vascular smooth muscle studied by 13CNMR.  J. Mol. Cell. Cardiol. 24:S25.

45)  Hardin, C.D., Wiseman, R.W., and Kushmerick, M.J. (1992).  Tension responses of sheep aorta to simultaneous decreases in Pi, PCr, and ATP.  J. Mol. Cell. Cardiol. 24:S24.

46)  Hardin, C.D., Wiseman, R.W., and Kushmerick, M.J. (1992).  The role of phosphorous metabolites in alterations of vascular tone.  Society of Magnetic Resonance in Medicine XIth Annual Meeting,  Berlin, Germany.

47)  Hardin, C.D., Wiseman, R.W., and Kushmerick, M.J. (1991). Multiple controls of vascular oxidative metabolism.  Society of Magnetic Resonance in Medicine Xth Annual Meeting. San Francisco, California.

48)  Hardin, C.D., and Kushmerick, M.J.  (1991).  Glucose metabolism and glycogen turnover in vascular smooth muscle studied by 13C-NMR.  J. Mol. Cell. Cardiol. 23:512.

49)  Wiseman, R.W., Hardin, C.D., Kushmerick, M.J., and Dykens, J.A. (1991).  31P-NMR assessment of specific impairment of creatine kinase function following hypoxia: implications for reperfusion injury.  FASEB J. 5(5):A5255.

50)  Zhang, C., Hardin, C.D., Paul, R.J., and Kranias, E.G. (1991).  Mechanisms of calmodulin activation of Ca2+-uptake in plasmalemmal vesicles of porcine antral smooth muscle.  FASEB J. 5(5):A7937.

51)  Hardin, C.D., and Kushmerick, M.J. (1990).  Glycogen turnover in vascular smooth muscle studied by 13C-NMR.  Society of Magnetic Resonance in Medicine IXth Annual Meeting. New York, NY.

52)  Hardin, C.D., Wiseman, R.W., and Kushmerick, M.J.  (1990).  Control of oxygen consumption in sheep aorta.  FASEB J.  4(3): A270.

53)  Hardin, C.D., Raeymaekers, L., Casteels, R., and Paul, R.J.  (1988).  Comparison of endogenous and exogenous sources of ATP in supporting Ca2+-uptake in isolated smooth muscle plasma membrane vesicles (PMV).  Biophys. J. 53(2):347.

54)  Hardin, C.D., Kranias, E.G., Raeymaekers, L., Steenart N.A.E., and Paul, R.J. (1988).  Phosphorylation of a smooth muscle plasma membrane vesicle fraction is associated with an increased glycolytically supported calcium uptake.  FASEB J. 2(4):A755.

55)  Hardin, C.D. (1987).  Myofibril bound fructoaldolase has an apparent equilibrium constant different than that measured in vitro.  Biophys. J. 51(2):310.

56) Hardin, C.D., Raeymaekers, L., Wuytack, F., Casteels, R., and Paul, R.J. (1987).  An endogenous glycolytic cascade can preferentially fuel Ca2+-uptake in a plasma membrane vesicle preparation (PMV) of smooth muscle.  Fed. Proc. 46(4):1096.

57) Paul, R.J., Hardin, C.D., Wuytack, F., Raeymaekers, L., and Casteels, R.  (1987). Glycolysis can fuel Ca2+-uptake in a plasma membrane vesicular (PMV) preparation of smooth muscle.  Biophys. J. 51(2):181.

58)  Hardin, C.D., and Paul, R.J.  (1986).  Association of glycolytic enzymes with chemically skinned guinea pig taenia coli.  Fed. Proc.  45:767.

 

Back Home